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Basic histological staining methods (preview) - Human Histology | Kenhub DrPhil 179 Views • 3 years ago

In order to be able to look at tissues under a microscope, we need to first stain them with the right technique. Learn the main staining techniques used in histology today on our full video: https://khub.me/aux9w

Oh, are you struggling with learning anatomy? We created the ★ Ultimate Anatomy Study Guide ★ to help you kick some gluteus maximus in any topic. Completely free. Download yours today: https://khub.me/e0th1

As you probably know, histology is the study of the microscopic anatomy of cells and tissues. So we use staining methods to visualize and distinguish the different parts of cells and tissues since cells and their structures are usually transparent or colorless. The types of dyes used to color cells and their components can either be specific to particular structures, chemical groups or even molecules, and it can also be non-specific in which case most of the cell is stained in the same way.

When staining tissue samples, dyes that are used are either acidic or basic or a combination of the two. And why is that, you might be asking. Well, cellular structures such as nucleic acids or proteins have charged groups which are known as phosphate groups or carboxyl groups, just to name a couple. The dyes used in histology are colored organic compounds which also have a charge. Acidic dyes carry a negative charge and so they bind to positively-charged cell structures.

In the full version of this tutorial, we will cover some of the most common types of dyes used in histological staining of cells and their structures:
- basic dyes vs acidic dyes vs neutral dyes;
- hematoxylin and eosin;
- PAS - staining;
- Golgi method;
- Toluidine blue;
- Masson's trichrome;
- Osmium tetroxide;

To master this topic, click on the link and carry on watching the full video (available to Premium members): https://khub.me/aux9w !

Want to test your knowledge on the different types of cells and tissues? Take this quiz: https://khub.me/3g19f

Read more on how to interpret different histological sections on this complete article which goes through the different stains used in histology https://khub.me/saimh

For more engaging video tutorials, interactive quizzes, articles and an atlas of Human anatomy and histology, go to https://khub.me/pkvz2

Muscle Tissues and Sliding Filament Model DrPhil 252 Views • 3 years ago

Join the Amoeba Sisters a they explore different muscle tissues and then focus on the sliding filament theory in skeletal muscle! This video also briefly talks about muscle naming, some vocabulary (such as agonists and antagonists) before focusing on the sliding filament model. Video also mentions general roles of tropomyosin and troponin.
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Table of Contents:
00:00 Intro
0:39 Muscle Tissue Types
1:58 Muscle Characteristics
2:33 Skeletal Muscle Naming and Arrangement
3:26 Actin Myosin and Sarcomere
4:32 Sliding Filament Model
6:55 Tropomyosin an Troponin
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Factual References:

Betts, J. Gordon, et al. “10.3 Muscle Fiber Contraction and Relaxation - Anatomy and Physiology 2e | OpenStax.” Openstax.org, 20 Apr. 2022, openstax.org/books/anatomy-and-physiology-2e/pages/10-3-muscle-fiber-contraction-and-relaxation.

Urry, Lisa A, et al. Campbell Biology. 11th ed., New York, Ny, Pearson Education, Inc, 2017.
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Further Reading Recommendations:

What about I and A bands? What actually initiates the power stroke? How does calcium get released and from where? Remember, there is a lot more detail! We recommend this page from Openstax to learn more:
https://openstax.org/books/bio....logy-2e/pages/38-4-m

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Spanish Subtitles Translated by Jeremy García
Hindi Subtitles: Translated by Alisha Aggarwal

We gladly accept subtitle translations from our community. Learn more here: https://www.amoebasisters.com/....pinkys-ed-tech-favor We want to thank our amazing community for the generosity of their time in continuing to create translated subtitles.

We also have videos dubbed in Spanish and Portuguese using an artificial voice via https://aloud.area120.google.com to increase accessibility. See our Amoeba Sisters en Español channel https://www.youtube.com/channe....l/UC1Njo3LBy53cOPngz and Amoeba Sisters em Português https://www.youtube.com/channe....l/UCYTQPX2X_mXe0ZMPi

Craniotomy and Craniectomy Scott 341 Views • 3 years ago

http://www.nucleushealth.com/ - This 3D medical animation depicts two operations, called craniotomy and craniectomy, in which the skull is opened to access the brain. The normal anatomy of the skull and tissues surrounding the brain are shown, including arteries and veins. The animation lists the common reasons for these procedures, and briefly introduces intracranial pressure.

Video ID: ANH13109

Transcript:

Your doctor may recommend a craniotomy or a craniectomy procedure to treat a number of different brain diseases, injuries, or conditions.

Your skull is made of bone and serves as a hard, protective covering for your brain. Just inside your skull, three layers of tissue, called meninges, surround your brain. The thick, outermost layer is the dura mater. The middle tissue layer is the arachnoid mater and the innermost layer is the pia mater. Between the arachnoid mater and the pia mater is the subarachnoid space, which contains blood vessels and a clear fluid called cerebrospinal fluid. Blood vessels, called bridging veins, connect the surface of your brain with the dura mater. Other blood vessels, called cerebral arteries, bring blood to your brain.

Inside your skull, normal brain function requires a delicate balance of pressure between the blood in your blood vessels, the cerebrospinal fluid that surrounds your brain, and your brain tissue. This is called normal intracranial pressure. Increased intracranial pressure may result from: brain tumors, head injuries, problems with your blood vessels, or infections in your brain or spinal cord. These conditions put pressure on your brain and may cause it to swell or change shape inside your skull, which can lead to serious brain injury.

Your doctor may recommend a craniotomy to remove: abnormal brain tissue, such as a brain tumor, a sample of tissue by biopsy, a blood clot, called a hematoma, excess cerebrospinal fluid, or pus from an infection, called an abscess.

A craniotomy may also be done to: relieve brain swelling,
stop bleeding, called a hemorrhage, repair abnormal blood vessels, repair skull fractures, or repair damaged meninges.

Finally, a craniotomy may also be done to: treat brain conditions, such as epilepsy, deliver medication to your brain, or implant a medical device, such as a deep brain stimulator.

The most common reason for a craniotomy is to remove a brain tumor.

#Craniotomy #Craniectomy #BrainSurgery

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