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Normal Skin Histology - Explained by a Dermatopathologist DrPhil 86 Views • 2 years ago

A complete organized library of all my videos, digital slides, pics, & sample pathology reports is available here: https://kikoxp.com/posts/5084 (dermpath) & https://kikoxp.com/posts/5083 (bone/soft tissue sarcoma pathology)

Topics discussed:

Epidermis:
Layers of epidermis: 0:10
Melanocytes vs Keratinocytes: 5:16
Langerhans cells: 10:10 & 33:30 & 57:30

Dermis:
Papillary and reticular dermis: 11:50
Three types of white empty spaces on a slide: vessels, glands/ducts/cysts, or artifact: 15:25
Blood vessels & nerves: 18:24 & 48:50 & 58:59
Arrector pili & other dermal smooth muscle: 20:00

Adnexal:
Sebaceous gland: 21:10
Hair follicle 23:14
Eccrine sweat glands and ducts 24:45 & 50:00
Gland/duct vs blood vessel 27:20 & 48:50
Apocrine glands: this video https://kikoxp.com/posts/7837 (at 12:30)
Acrosyringium: this video https://kikoxp.com/posts/7837 (at 10:00)

Three types of pink bundles: smooth muscle, nerve, dense connective tissue: 27:50

Acral skin (palm sole) with contact dermatitis 29:37
Parakeratosis 30:00
Perivascular lymphocytes 30:40
Eosinophils vs neutrophils 31:20
Spongiosis with desmosome keratinocyte spines 32:10
Spongiotic vesicles with Langerhans cells 33:30
Normal acral skin (palm & sole) with stratum lucidum 34:20
Normal glomus body/apparatus (canal of Sucquet-Hoyer) 35:40
Nerve 36:46 & 51:50
Adipose tissue (white fat cells) in subcutis with Lochkern 37:55
Normal scalp skin with large anagen hair follicles: 39:30
Hair follicle anatomy (bulb/matrix, inner root sheath, outer root sheath, hair shaft, isthmus, infundibulum): 40:55 (labeled images):
https://kikoxp.com/posts/3661 & https://kikoxp.com/posts/7899
Pacinian corpuscle 50:40
Meissner corpuscle 1:02:28

Dense regular connective tissue (Fascia/Tendon/Ligament) vs Smooth Muscle 53:00

Basic Normal Skin Immunohistochemistry:
-cytokeratin in epidermis: 55:33
-S100 in melanocytes and Langerhans cells and adipocytes: 57:30
-Desmin in smooth muscle (arrector pili and blood vessels): 58:59
-CD31 in endothelial cells of blood vessels: 59:33
-SOX-10 in melanocytes: 1:00:40

Digit/Finger/Toe histology (amputation for subungual acral melanoma) 1:04:10 & 1:08:30
-bone 1:05:40
-glomus body 1:05:15
-tendon/ligament 1:06:10
-artery 1:06:58
-fingernail/toenail 1:08:54
-acrosyringium 1:10:45

Solar elastosis (what wrinkles look like microscopically!) 1:11:50

Other videos you might like:
Tendon vs Nerve Histology Made Simple with the Ramen Noodle Sign (of Fulton) video: https://kikoxp.com/posts/4466
Melanocytes vs Keratinocytes made easy video: https://kikoxp.com/posts/3802
Blood Vessel vs Gland vs Artifact Made Easy video: https://kikoxp.com/posts/4808

The basic normal structures of the skin discussed and described by a dermatopathologist. This material is intended for use by medical students, junior pathology or dermatology residents, or for anyone else studying normal human histology. Special thanks to two of my medical students at UAMS for helping make this video possible. Miki Lindsey convinced me that I really needed to sit down and record this video. Akash Patel took time to edit the video and make it ready for YouTube. My sincere thanks to both of them for helping me overcome procrastination.

Huge thanks to Abigail Cline, a medical student at Medical College of Georgia, for volunteering to type a transcript of this ENTIRE video (over 14,000 words!) so that I could provide closed caption subtitles for those with hearing impairments and for those who may need assistance in understanding spoken English (particularly given how quickly I speak!). You can access a text version of her transcript of my video here: https://kikoxp.com/posts/5390

Correction - I made a mistake in the video. I said that sebaceous gland secretions are turned into smelly substances by bacteria and that this makes body odor. That is incorrect. That is actually true of APOCRINE gland secretions not sebaceous secretions.

Also, in the past I used "keratinocyte" and "squamous cell" interchangeably (this is because in dermatopathology, we see and talk about squamous cell carcinomas all the time, and those tumors are composed of keratinocytes). But technically, in normal skin histology, "squamous cell" refers only to the flattened keratinocytes in the superficial epidermis. Thankfully, a histology PhD colleague pointed this out to me and corrected my lazy nomenclature!

Please check out my Soft Tissue Pathology & Dermatopathology survival guide textbooks: http://bit.ly/2Te2haB ‬

This video is geared towards medical students, pathology or dermatology residents, or practicing pathologists or dermatologists. Of course, this video is for educational purposes only and is not formal medical advice or consultation.

Presented by Jerad M. Gardner, MD. Please subscribe to my channel to be notified of new pathology teaching videos.

Follow me on:
Snapchat: JMGardnerMD
Twitter: @JMGardnerMD
Instagram: @JMGardnerMD
Facebook: https://www.facebook.com/JMGardnerMD/

MCL tear of the knee: Injury, diagnosis, treatment Scott 85 Views • 2 years ago

We are looking for 5 patients with knee pain who want to get significantly better in the next 30 days. Click this link to let me know you're interested and learn more.

https://www.drdavidgeier.com/work-with-me/contact/

One of the most common knee injuries in contact and collision sports is a medial collateral ligament (MCL) injury. This is a ligament on the medial (side closest to the midline) side of your knee that provides stability against side-to-side stress to the knee. You might injure it by cutting maneuvers in sports like soccer or hockey. You can also suffer an MCL injury if another player hits you on the outside of your knee.

Please note: I don't respond to questions and requests for specific medical advice left in the comments to my videos. I receive too many to keep up (several hundred per week), and legally I can't offer specific medical advice to people who aren't my patients (see below). If you want to ask a question about a specific injury you have, leave it in the comments below, and I might answer it in an upcoming Ask Dr. Geier video. If you need more detailed information on your injury, go to my Resources page: https://www.drdavidgeier.com/resources/

The content of this YouTube Channel, https://www.youtube.com/user/drdavidgeier (“Channel”) is for INFORMATIONAL PURPOSES ONLY. The Channel may offer health, fitness, nutritional and other such information, but such information is intended for educational and informational purposes only. This content should not be used to self-diagnose or self-treat any health, medical, or physical condition. The content does not and is not intended to convey medical advice and does not constitute the practice of medicine. YOU SHOULD NOT RELY ON THIS INFORMATION AS A SUBSTITUTE FOR, NOR DOES IT REPLACE, PROFESSIONAL MEDICAL ADVICE, DIAGNOSIS, OR TREATMENT. You should consult with your healthcare professional before doing anything contained on this Channel. You agree that Dr. Geier is not responsible for any actions or inaction on your part based on the information that is presented on the Channel. Dr. David Geier Enterprises, LLC makes no representations about the accuracy or suitability of the content. USE OF THE CONTENT IS AT YOUR OWN RISK.

Unlike tears of the ACL, MCL injuries most often heal without surgery. You might need to wear a hinged knee brace for 2-6 weeks. The length of time you miss from sports or exercise varies depending on the location and severity of the injury.

In this video, I share my thoughts on the nature of an MCL injury, the diagnosis, the treatment options and return to sports.

Please remember, while I appreciate your questions, I cannot and will not offer specific medical advice by email, online, on my show, or in the comments at the end of these posts. My responses are meant to provide general medical information and education. Please consult your physician or health care provider for your specific medical concerns.

Histology of Dentin DrPhil 84 Views • 2 years ago

The dentin is a hard tissue that forms the bulk of the tooth. It is similar to bone but is slightly harder, although softer than enamel. The dentin has numerous dentinal tubules that run across its length. Each dentinal tubule houses the cytoplasmic process of an odontoblast (odontoblastic process).
📄Notes for the video: https://www.hackdentistry.com/....bundles/revision-nin

💻Website: https://www.hackdentistry.com/
📰Blog: https://hackdentistry.substack.com/

Study resources on our website-
📖Oral pathology Revision Ninja (Notes, Videos & MCQs): https://www.hackdentistry.com/bundles/oral-pathology-revision-ninja
📖Oral Histology Revision Ninja (Notes, Videos & MCQs): https://www.hackdentistry.com/....bundles/revision-nin
📖Periodontics Revision Ninja (Notes & MCQs): https://www.hackdentistry.com/bundles/perio-rn
📖Question Bank: https://www.hackdentistry.com/bundles/question-bank
📖Access all content: https://www.hackdentistry.com/bundles/all-access-premium

References and further reading:
💡Berkovitz BKB, Hollan GR, Moxham BJ. Oral Anatomy, Histology and Embryology. 4th ed. Mosby Elsevier; 2009.
💡Nanci A. Tencate’s Oral Histology. Development, Structure and Function. 8th ed. Elsevier; 2013.
💡Kumar GS. Orban’s Oral Histology and Embryology.13th ed. Elsevier; 2011.
💡Avery JK. Oral development and Histology. 3rd ed. Thieme Medical Publishers; 2002.

Log in to https://www.hackdentistry.com and get access to:
I) Numerous Notes/Cheatsheets and Videos II) Thousands of quiz questions from our vast Question Bank!

HackDentistry is an edtech company that aims make learning dentistry fun,engaging and light hearted.
1) It focuses on helping students understand and retain core concepts in dentistry through highly visual sketch/whiteboard style video animations.
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3) It also provides for a community platform where students can come together, and engage with fellow dental students and dentists across the globe!

Facebook:
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Instagram:
https://www.instagram.com/hack.dentistry/

Twitter:
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Basic histological staining methods (preview) - Human Histology | Kenhub DrPhil 83 Views • 2 years ago

In order to be able to look at tissues under a microscope, we need to first stain them with the right technique. Learn the main staining techniques used in histology today on our full video: https://khub.me/aux9w

Oh, are you struggling with learning anatomy? We created the ★ Ultimate Anatomy Study Guide ★ to help you kick some gluteus maximus in any topic. Completely free. Download yours today: https://khub.me/e0th1

As you probably know, histology is the study of the microscopic anatomy of cells and tissues. So we use staining methods to visualize and distinguish the different parts of cells and tissues since cells and their structures are usually transparent or colorless. The types of dyes used to color cells and their components can either be specific to particular structures, chemical groups or even molecules, and it can also be non-specific in which case most of the cell is stained in the same way.

When staining tissue samples, dyes that are used are either acidic or basic or a combination of the two. And why is that, you might be asking. Well, cellular structures such as nucleic acids or proteins have charged groups which are known as phosphate groups or carboxyl groups, just to name a couple. The dyes used in histology are colored organic compounds which also have a charge. Acidic dyes carry a negative charge and so they bind to positively-charged cell structures.

In the full version of this tutorial, we will cover some of the most common types of dyes used in histological staining of cells and their structures:
- basic dyes vs acidic dyes vs neutral dyes;
- hematoxylin and eosin;
- PAS - staining;
- Golgi method;
- Toluidine blue;
- Masson's trichrome;
- Osmium tetroxide;

To master this topic, click on the link and carry on watching the full video (available to Premium members): https://khub.me/aux9w !

Want to test your knowledge on the different types of cells and tissues? Take this quiz: https://khub.me/3g19f

Read more on how to interpret different histological sections on this complete article which goes through the different stains used in histology https://khub.me/saimh

For more engaging video tutorials, interactive quizzes, articles and an atlas of Human anatomy and histology, go to https://khub.me/pkvz2

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