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Basic histological staining methods (preview) - Human Histology | Kenhub DrPhil 83 Views • 2 years ago

In order to be able to look at tissues under a microscope, we need to first stain them with the right technique. Learn the main staining techniques used in histology today on our full video: https://khub.me/aux9w

Oh, are you struggling with learning anatomy? We created the ★ Ultimate Anatomy Study Guide ★ to help you kick some gluteus maximus in any topic. Completely free. Download yours today: https://khub.me/e0th1

As you probably know, histology is the study of the microscopic anatomy of cells and tissues. So we use staining methods to visualize and distinguish the different parts of cells and tissues since cells and their structures are usually transparent or colorless. The types of dyes used to color cells and their components can either be specific to particular structures, chemical groups or even molecules, and it can also be non-specific in which case most of the cell is stained in the same way.

When staining tissue samples, dyes that are used are either acidic or basic or a combination of the two. And why is that, you might be asking. Well, cellular structures such as nucleic acids or proteins have charged groups which are known as phosphate groups or carboxyl groups, just to name a couple. The dyes used in histology are colored organic compounds which also have a charge. Acidic dyes carry a negative charge and so they bind to positively-charged cell structures.

In the full version of this tutorial, we will cover some of the most common types of dyes used in histological staining of cells and their structures:
- basic dyes vs acidic dyes vs neutral dyes;
- hematoxylin and eosin;
- PAS - staining;
- Golgi method;
- Toluidine blue;
- Masson's trichrome;
- Osmium tetroxide;

To master this topic, click on the link and carry on watching the full video (available to Premium members): https://khub.me/aux9w !

Want to test your knowledge on the different types of cells and tissues? Take this quiz: https://khub.me/3g19f

Read more on how to interpret different histological sections on this complete article which goes through the different stains used in histology https://khub.me/saimh

For more engaging video tutorials, interactive quizzes, articles and an atlas of Human anatomy and histology, go to https://khub.me/pkvz2

Why Your Knee Hurts. Knee Pain Types By Location & Description. Scott 81 Views • 2 years ago

Knee pain location can often tell you what type of knee pain you have. If you confirm that with common symptoms and what aggravates it… you can get a pretty good idea of ‘why my knee hurts’. So, here’s a quick look at the most common type of knee problems.

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0:00 Intro
0:11 Pain at the front of the knee (Pain in kneecap)
0:42 Pain below kneecap
1:40 Pain on inside of knee
3:05 Pain below knee on inside
3:29 Pain on outside of knee
3:28 Pain above knee
3:28 Pain behind knee

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Mentioned in this video...

How To Fix Pain In The Front Of The Knee… (Runner's Knee) https://youtu.be/g0qmx_0enAA

Looking to stop your knee problems? Do this...

Knee Strengthening Exercises To Prevent Knee Pain
https://youtu.be/Pk-ae_lyx7M

How To Treat Patellar Tendinopathy (Jumper’s Knee) & Quadriceps Tendinopathy
https://youtu.be/MkPwsb-rQwU
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If you’re asking yourself ‘what’s the cause of my knee pain?’ or ‘what kind of knee pain do I have?’ (so that you can look up solutions to your knee pain on YouTube) the position of your knee pain will tell you a lot.

THE MOST COMMON KNEE PAIN TYPES?
Knee pain during running (or actually kneecap pain while running) is usually just that… Runner’s Knee (PATELLOFEMORAL PAIN SYNDROME, or it’s old name: Chondromalacia Patella) If you get knee pain while cycling it will often be the same thing. Same with knee pain with stairs.

Knee pain while squatting could be Runner’s Knee, but if you get pain in the tendon below the kneecap, it’s more likely to be Patellar Tendonitis or Jumper’s Knee.

Meniscus Tears will give you pain on the inside of the knee that is a localised pain, can feel as if it gets stuck, or feel like it’s going to give way, and often it’s hard to fully straighten or fully bend your knee.

Knee pain on the outside of the knee is usually Iliotibial Band Syndrome

ALSO COVERED:
Infrapatellar Fat Pad Syndrome (Hoffa's Syndrome)
Osgood-Schlatter Disease
Medial Collateral Ligament Tear
Iliotibial Band Syndrome
Osteoarthritic Knee Pain
Pes Anserine Bursitis.
Quadriceps Tendinopathy
Popliteus Strain
Baker’s Cyst
ACL Or PCL Tear/Rupture

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#bodyfixexercises #kneepainrelief #kneepain

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