Stool tests (FIT, culture, calprotectin)

Category: Biopsies, Lab & Pathology Tests · All biopsies, lab & pathology tests

Stool tests (FIT, culture, calprotectin) — Biopsies, Lab & Pathology Tests

What it is

Stool tests include FIT (faecal immunochemical test — detects hidden blood), culture and PCR panels (find infection organisms), calprotectin (a marker of bowel inflammation), and tests for parasites and toxins such as C. difficile.

Why it is done

FIT is used in bowel cancer screening programmes and to assess people with bowel symptoms at lower risk of cancer. Calprotectin helps distinguish inflammatory bowel disease (IBD) from irritable bowel syndrome (IBS). Cultures investigate infectious diarrhoea.

How to prepare

Your kit comes with instructions: a small sample is collected with the provided stick or spoon into the container — the FIT needs only a tiny scrape. Avoid contamination with toilet water where instructed. Note recent antibiotics and travel for culture requests.

What happens during the procedure

In the laboratory, FIT measures human haemoglobin in the sample quantitatively; calprotectin is extracted and measured by immunoassay; cultures and PCR identify organisms. No procedure on you is involved — you provide the sample at home or in clinic.

Afterwards — recovery & results

Results take a few days typically (FIT screening results by post or portal). A positive FIT leads to colonoscopy referral in screening and symptomatic pathways. Your clinic explains your individual results and next steps.

Risks & considerations

There are no risks to you. The commonest issue is sampling error — follow the kit instructions carefully and return the sample promptly as directed.

Frequently asked questions

A positive FIT means cancer?

No — most positive FITs are not cancer; blood can come from polyps, inflammation, haemorrhoids and other causes. It means the bowel needs a proper look with colonoscopy.

Why calprotectin instead of going straight to colonoscopy?

In younger patients with typical IBS symptoms and a low calprotectin, colonoscopy can often be avoided safely. A high result prioritises endoscopy and IBD assessment.

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Please note: This page is educational information about a medical procedure or test. It does not replace the instructions of your own care team, who know your situation and whose preparation, fasting and medication advice you should follow if it differs from anything here. If you have symptoms that worry you, or an appointment you do not understand, contact your clinical team.